Modifying the Cell Painting Assay - AskErinDearBeth Episode 30

In this episode, Erin and Beth talk about the Cell Painting Assay and how you can modify it. The TLDR is - do what you want, it doesn't really matter! The strength is in the technique of morphological profiling and the Cell Painting Assay is a great assay for morphological profiling because of how cheap and easy it is. You'll want to have a background understanding of regular, arrayed Cell Painting, so if you don't, watch this episode first: Introduction to the Cell Painting Assay -    • Introduction to the Cell Painting Assay - ...   And if you want to deep dive into Cell Painting, check out these episodes: Interpreting Cell Painting Features: Classic Cell Profiler Features -    • Interpreting Cell Painting Features: Class...   Read the most recent Cell Painting Protocol paper (http://doi.org/10.1038/s41596-023-008...) for quantification of how modifications affect the strength of the assay and Assessing the performance of the Cell Painting assay across different imaging systems (https://doi.org/10.1002/cyto.a.24786) for further modifications. Look in the Cell Painting wiki (https://github.com/carpenter-singh-la...) for some of the modifications that were tested in the development of the Cell Painting Assay. The Cell Painting Gallery hosts a LOT of Cell Painting data. You can find many examples of published datasets using a variety of modified Cell Painting protocols in the Cell Painting Gallery (https://github.com/broadinstitute/cel.... #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #segmentation #cellprofiler ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/FIJI, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...