Enzymes Kinetics - Episode 02 | Biochemistry #csirnetlifescience #biotechnology #icmrjrf #gateexam

Enzyme Series – Part 2 | Enzyme Kinetics | Biochemistry Series Welcome to Part 2 of the Enzyme Series, a sub-series of our Biochemistry Series, where we dive deeper into the fundamentals of enzyme kinetics. This lecture is especially useful for students preparing for CSIR NET Life Sciences, GATE XL, DBT BET, TIFR JGEEBILS, and other Life Science competitive examinations. In this lecture, we understand how the concentration of substrate affects enzyme reaction velocity and explore the important parameters used to describe enzyme activity and efficiency. 📚 Topics Covered in This Video: 🔹 Order of Reactions Zero-order reactions First-order reactions Second-order reactions Relationship between substrate concentration and reaction velocity 🔹 Michaelis-Menten Kinetics Enzyme-substrate complex Substrate saturation Initial velocity Michaelis-Menten model Michaelis-Menten equation Understanding the assumptions behind the model 🔹 Km – Michaelis Constant Concept and significance of Km Relationship between Km and substrate concentration Km and ½ Vmax Interpretation of high and low Km 🔹 Kd and Ka Dissociation constant (Kd) Association constant (Ka) Relationship between binding affinity and these constants Difference between Km and Kd 🔹 Vmax – Maximum Velocity Concept of enzyme saturation Maximum reaction velocity Relationship between enzyme concentration and Vmax 🔹 Michaelis-Menten Graph Reaction velocity vs. substrate concentration Hyperbolic curve Vmax ½ Vmax Determination of Km from the graph 🔹 Turnover Number (kcat) Definition of kcat Turnover number equation Relationship between kcat and Vmax Catalytic capacity of an enzyme 🔹 Catalytic Efficiency kcat/Km Comparing enzyme efficiency Relationship between catalytic speed and substrate concentration 🔹 Specific Activity Definition of specific activity Enzyme activity per amount of protein Importance of specific activity in enzyme purification Difference between enzyme activity and specific activity 🎯 Exam Focus: The lecture focuses on understanding the concepts behind the equations, graphs, and parameters, rather than simply memorizing formulas. These concepts are extremely important for solving CSIR NET Part B & Part C, GATE XL, DBT BET, and other enzyme-kinetics questions. 🚀 Coming Next: Advanced enzyme kinetics, enzyme inhibition, Lineweaver-Burk plots, competitive and non-competitive inhibition, and more exam-oriented problems. 📌 Watch Part 1 first to understand the introduction to enzymes, enzyme classes, mechanism of enzyme action, thermodynamics, and activation energy.