Coagulase Test - Amrita University

▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @    / amritacreate      / amritavlab   ▶ Like us @   / createatamrita   ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=... ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Coagulases are enzymes that clot blood plasma by a mechanism that is similar to normal clotting. The coagulase test identifies whether an organism produces this exoenzyme. The coagulase test is used to differentiate from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase). Bound coagulase otherwise known as "Clumping factor" can be detected by carrying out a slide coagulase test and free coagulase can be detected by doing a tube coagulase test. Coagulase can tightly bound to the surface of the S. aureus and coat its surface with fibrin upon contact with blood. It is also proposed fibrin-coated staphylococci resist phagocytosis thereby increasing the virulence capabilty of the bacteria.