Evaluation of cell viability ― Using CellTiter-Glo 3D Cell Viability Assay and P3D Scaffolds

Video protocol for how to evaluate cell viability ― Using CellTiter-Glo 3D Cell Viability Assay and P3D Scaffolds from @Ossiform Research Line. Content: *Note: See protocol for important procedures before you start. 00:14 - If necessary, remove media from wells to achieve volumes listed in table in the protocol 00:24 - Add Assay Reagent to each well, using multichannel pipette if possible 01:21 - Place plate at RT on a plate shaker for 5 min. 01:40 - Remove plate from plate shaker and incubate plate at RT for 25 min. 01:54 - Pipette 200 uL lysate to opaque plate as quickly as possible 02:17 - Measure luminescence using a plate reader with integration time 0,25-1 seconds Make sure to check our latest updated protocols on our website: https://ossiform.com/ossiform-researc...