Applications of the Fluorescent Amino Acid Acridonylalanine

Talk given by James Petersson (University of Pennsylvania) as part of the International GCE Webinar series. Live talk given on May 18th, 2023. The fluorescent labeling of proteins has tremendous value for in vitro biophysical experiments as well as for cell-based imaging. In all of these experiments, incorporation of intrinsically fluorescent non-canonical amino acids via GCE can provide significant advantages over labeling using fluorescent protein fusions or chemical modification. I will discuss these advantages as I describe the incorporation of acridonylalanine (Acd) through GCE in bacteria for biophysical analysis of purified proteins and in mammalian cells for imaging. A variety of applications will be described, using Acd alone and in combination with FRET partners.