I2KxBINA 2026 Community Congress | Napari-phasors: Interactive & Unified Framework - Bruno Pannunzio

The following presentation was given during I2KxBINA's 2026 Community Congress: Cultivating the Multiscale Imaging Frontier. Bruno Pannunzio - Napari-phasors: An Interactive and Unified Framework for FLIM and Hyperspectral phasor Analysis This workshop provides a hands-on introduction to napari-phasors, an open-source tool designed for the unified analysis of FLIM and hyperspectral data within the napari ecosystem. Participants will learn to navigate a complete workflow, from importing raw formats to performing multi-harmonic calibration and interactive species selection. The session emphasizes practical quantification, demonstrating how to transform multidimensional images into statistical distributions and publication-quality visualization plots. By mastering these tools, attendees will unlock the full potential of the phasor approach to resolve complex biological environments through an intuitive and fit-free analytical framework. The plugin provides a streamlined workflow starting from the direct import of diverse raw file formats such as .ptu, .sdt, and .lsm. We demonstrate its ability to perform simultaneous multi-layer analysis, enabling the management of complex experimental conditions in a single session. Beyond multi-harmonic calibration, filtering and pixel-wise mapping of lifetime, phase, and modulation, napari-phasors features a robust statistical framework. The results of the analysis distribution can be interactively visualized through integrated histograms and statistics tables, creating a complete workflow that spans from raw data to quantitative visualization plots. With specialized features like FRET and component analysis, napari-phasors provides a high-performance, accessible solution for the broader scientific community. Fluorescence Lifetime Imaging (FLIM) and Hyperspectral Imaging are essential for resolving complex biological environments, yet extracting quantitative insights from these multidimensional datasets is often hindered by a fragmented software landscape. Despite the power of the phasor approach, there is a notable lack of open-source, interactive, and user-friendly tools that combine phasor analysis for both FLIM and hyperspectral data within a single interface. To address this, we present napari-phasors, a comprehensive plugin built upon the PhasorPy library. By integrating these capabilities into the napari multidimensional viewer, users can leverage the vast ecosystem of existing napari tools, such as advanced segmentation masks and community-driven plugins, creating a synergistic environment for sophisticated bioimage analysis. Some relevant resources for this presentation for youtube viewers: https://napari-phasors.readthedocs.io... https://napari-phasors.readthedocs.io... https://github.com/napari-phasors/nap...