Webinar: Understanding mechanisms of drugs toxicity using hepatocytes-based spheroids.

Prof. By Adelina Rogowska-Wrzesinska, University of Southern Denmark In this presentation, I will discuss how we use HepG2/C3A spheroids to study hepatocytes response to therapeutic-equivalent doses of APAP (5 mg APAP per mg total soluble protein). I will share tips and tricks on how to design experiments to obtain maximum sensitivity and reproducibility. I will present how advanced mass spectrometric techniques was used to quantify changes in proteins and their S-nitrosylation and S-sulfenylation levels in C3A spheroids treated with APAP, and how this approach is explored to characterize the early-stage drug response that is very often overlooked in rodent based toxicity models.