9- High Performance Liquid Chromatography (HPLC) [𝐈𝐧𝐬𝐭𝐫𝐮𝐦𝐞𝐧𝐭𝐚𝐥 𝐀𝐧𝐚𝐥𝐲𝐬𝐢𝐬 𝐈]

This lecture provides a comprehensive introduction to High Performance Liquid Chromatography (HPLC), one of the most powerful and widely employed separation techniques in modern Analytical Chemistry. The content is intended for undergraduate and postgraduate students studying Pharmacy, Chemistry and Pharmaceutical Sciences. Topics covered in this lecture include: Principles of High Performance Liquid Chromatography (HPLC) Theory of chromatographic separation Mobile phase and stationary phase Instrumentation and system components Solvent delivery systems (HPLC pumps) Sample injection techniques Chromatographic columns and stationary phases HPLC detectors (UV-Visible, Fluorescence, Refractive Index, and others) Chromatogram interpretation Retention time, peak area, and peak resolution Factors influencing chromatographic separation Method optimization and analytical performance Applications of HPLC in pharmaceutical analysis, quality control, clinical laboratories, environmental monitoring, and scientific research #highperformanceliquidchromatography #hplc For Contact: 📍 Facebook personal account:   / mostafa.elnaggar.14224   📍 Youtube channel:    / @mostafanaggar-ie4jn   📍 Email address: mostafa.elnaggar@alexu.edu.eg 📍 Mobile (Whatsapp): +20 10 12463029 📍 Facebook group: https://www.facebook.com/share/g/19XA... 📍 Tiktok account: https://www.tiktok.com/@user296141809...