Multipass CCS Refiner Demonstration

At present, no widely accessible tools exist to support multipass calibration of cIM-MS systems, thereby hindering the broader adoption of refined multipass CCS strategies. To address this limitation, we introduce the Multipass CCS Refiner, a custom R script equipped with a Shiny graphical user interface. This tool enables users to perform multipass CCS calibration following the Lin and Costello approach (https://pubs.acs.org/doi/10.1021/acs.analc...) regardless of programming expertise. The Multipass CCS Refiner streamlines the process of generating calibration curves and calculating CCS values from multipass data. Calibration curves can be tailored to specific experimental conditions using reference standards with known CCS values. Additionally, the application allows users to download example and result files to facilitate data uploading, processing, and visualization. The Multipass CCS Refiner is hosted online via Posit and can be accessed at: https://ericgier.shinyapps.io/Multipass-CC.... Complete documentation is available on GitHub: https://github.com/facundof2016/Multipass-.... The application was developed in the R programming language (version 4.5.0), using RStudio (version 2025.05.1), and leverages the R Shiny framework (version 1.10.0) to provide an interactive web-based interface We gratefully acknowledge the foundational work of the Lin and Costello group, whose calibration strategy provided the basis for this tool. We acknowledge support from NIH awards R01CA218664 and R61CA281667 and funding support from the Air Force Institute of Technology through the Civilian Institutions Program.