Rich n Chip’s Tips and Tricks for Best Quantitation and Kinetics Performance on a BLI system

Biolayer interferometry (BLI) is gaining popularity for protein and small molecule quantitation and kinetics research. Even though BLI is one of the easiest of the tools, as with many other techniques, getting the best data depends on optimization of some key experimental factors. This webinar discusses, with specific examples, the impact of these factors on the results and common pitfalls. Chapters 00:00 Introductions 01:51 About Gator Bio 05:08 Agenda Review 05:42 What is BLI? 09:15 Guidelines for all BLI Assays 10:46 Choose the right buffer 11:46 Choose the right probe 13:46 New AAVX probe 15:00 Quantitation basics 20:47 Kinetics basics 27:05 Non-Specific Binding 28:23 pM affinity interactions 30:03 Kinetics Analysis 36:04 Q&A session #kinetics #biolayerinterferometry #tutorial https://www.gatorbio.com https://en.wikipedia.org/wiki/Bio-lay...