Semiconservative DNA Replication | Meselson Stahl Experiment | NEET Class 12 | Neela Bakore
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Semiconservative DNA Replication | Meselson Stahl Experiment | NEET Class 12 | Neela Bakore
268 697 просмотров · 10 л. назад
Neela Bakore Tutorials
598 тыс. подписчиков
268 697 просмотров · 10 л. назад
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Watson and Crick predicted it — Meselson and Stahl proved it! This video covers the elegant experiment that confirmed semiconservative DNA replication.
📌 Topics Covered:
• Three models of replication: Semiconservative, Conservative, Dispersive
• Meselson and Stahl experiment (1958) using E. coli
• ¹⁵N (heavy) and ¹⁴N (light) isotopes of nitrogen
• CsCl density gradient ultracentrifugation
• Results after 1 generation: hybrid (intermediate) band
• Results after 2 generations: hybrid + light bands
• Why only semiconservative model fits these results
• NEET MCQ patterns on Meselson-Stahl
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Transcript with Timestamp
0:00 Semiconservative replication: one old strand + one new strand retained
1:13 Meselson & Stahl experiment using heavy nitrogen N15 in E. coli
2:30 N15 vs N14 separated by cesium chloride density gradient
3:06 Started with N15/N15 DNA → transferred to N14 medium
4:06 1st generation: N15/N14 hybrid DNA
4:49 2nd generation: hybrid + N14/N14 (light) DNA
7:44 Result: heavy → hybrid → hybrid + light (confirmed semiconservative)
9:16 Taylor's experiment: root tip cells of Vicia faba using tritiated thymidine
10:23 Both experiments confirmed semiconservative replication
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