Designing and Validating HPLC methods with Regulatory Confidence : Day 3

Day 3 covered: 1. Basics of HPLC (instrumentation, working and principle) - approx 5-10 mins 2. ⁠Chromatography principle and types 3. ⁠Theory (rate and plate, HETP, etc) 4. ⁠Basics of troubleshooting and why is it done, when is it done 5. ⁠Mechanism of failure (Chemical factors, mass transfer, van deemter equation) 6. ⁠symptoms based mapping (basics) - Tailing, fronting, Signal to noise etc 7. ⁠SST