Part 1: Gene splicing by overlap extension (SOE PCR).

This video outlines a method (including an example) for designing primers for gene knockout in a bacterial genome using the Gene Splicing by Overlap Extension (SOE PCR) method. More details of this method can be found in the paper by Horton et al., (2018). The NCBI primer BLAST program can be found at: https://www.ncbi.nlm.nih.gov/tools/pr...