Thresholding in CellProfiler Part 3: Correction Factor and Limits - AskErinDearBeth Episode 28

In this episode, Erin and Beth wrap up a 3-part series on Thresholding in CellProfiler with an exploration of Correction Factors and Thresholding Limits. Remember, when you're bowling, try not to hit the bumpers but let the bumpers save you when otherwise you would have ended up in the gutter! Other episodes in the series: Thresholding in CellProfiler Part 2: Picking the Right Algorithm -    • Thresholding in CellProfiler Part 2: Picki...   Thresholding in CellProfiler Part 1: Adaptive Thresholding for Uneven Illumination -    • Thresholding in CellProfiler Part 1: Adapt...   #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #segmentation #cellprofiler ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/FIJI, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...