March 2022: Modeling Congenital Heart Disease in Mice Through Ultrasound-Guided Microinjections

This is a recording of a LIVE webinar presented by Anum Rahman, MD-PhD Student in the Faculty of Medicine and Department of Medical Biophysics at the University of Toronto on March 1st, 2022. Topics discussed in this webinar will be: How to perform ultrasound-guided microinjections in fetal mice: surgical and imaging tips and tricks Evaluating the fetal heart with ultrasound and MRI Extending the developed methodology for probing disease mechanisms in other organ systems: modeling placental disease and stroke Q&A Session --------- For more information on our products, please visit https://www.visualsonics.com. 0:00 Introduction 2:25 Hypoplastic left heart syndrome (HLHS) 10:40 How to perturb blood flow in the mouse fetus? 12:28 Microinjection setup 13:46 Surgically induced mouse model of HLHS 20:41 Surgical setup 21:55 Outcomes at gestational term (E18,5) 22:21 Factors responsible for inaccurate embolization 24:05 All embolized fetuses have retrograde flow 25:36 All embolized fetuses have severe left heart hypoplasia 28:00 Half of embolized fetuses had distal fragmentation 28:50 Significance 33:30 Other applications - ischemic stroke/hemorrhagic stroke 35:23 Advantages of current method 36:19 Acknowledgement