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Webinar: Functional annotation and pathway analysis using OmicsBox

BioBam Bioinformatics

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Webinar: Functional annotation and pathway analysis using OmicsBox

967 просмотров · 7 месяцев назад
BioBam Bioinformatics
931 подписчик
967 просмотров · 7 месяцев назад
Learn more about OmicsBox: https://www.biobam.com/omicsbox/ Request a personalized OmicsBox demo here: https://www.biobam.com/book-a-demo/ Practical approaches for generating novel annotations and analyzing pathways of differentially expressed genes. This webinar demonstrates how to annotate and analyze sequence datasets. The session covers annotation strategies with Blast2GO and presents downstream analysis options, including enrichment analysis, pathway analysis, and popular visualization techniques. A practical OmicsBox demo shows how to use a standard RNA-Seq dataset to transform raw reads into biological insights. The video features key theoretical concepts alongside a step-by-step practical demonstration using OmicsBox. Key topics covered in this video: How to (re)annotate sequence datasets using the Blast2GO methodology. How to review and combine multiple sources like InterPro and EggNog. How to perform enrichment analyses, including GSEA and Fisher’s Exact Test. How to link and visualize expression data to altered pathways from databases like KEGG and Reactome. Speaker: Stefan Götz, PhD. (Author of Blast2GO) CHAPTERS: 00:00 - Welcome and Introduction 01:33 - Webinar Content Overview 03:17 - About BioBam & OmicsBox 19:15 - Transcriptomics & Functional Interpretation Pipeline 20:47 - Case Study 1: Drug Response in a Non-Model Organism 23:13 - Case Study 2: Developmental Transcriptomics 25:19 - Transcriptomics Pipeline Overview 32:49 - Functional Annotation Pipeline 39:52 - Combined Pathway Analysis Theory 43:46 - Demo: Analyzing Dataset 2 44:10 - Demo: Quantification and Differential Expression 48:28 - Demo: Loading Functional Annotation 50:04 - Demo: Functional Enrichment with GSEA 53:06 - Demo: Combined Pathway Analysis 01:01:45 - Demo: Notes on Dataset 1 01:03:43 - Summary & Take-Home Messages ADDITIONAL RESOURCES: OmicsBox User Manual & Documentation: https://help.biobam.com/ Read our Blog for more bioinformatics tips: https://www.biobam.com/blog/ Try OmicsBox for free: https://www.biobam.com/trial/ More about Functional Analysis in OmicsBox: https://www.biobam.com/functional-ana... CONNECT WITH BIOBAM: • Website: https://www.biobam.com • LinkedIn:    / biobam   • Twitter/X: https://x.com/bio_bam • Facebook:     / biobambioinformatics   #Bioinformatics #FunctionalAnnotation #Blast2GO #OmicsBox #PathwayAnalysis #GSEA #RNASeq #Transcriptomics