Webinar: Functional annotation and pathway analysis using OmicsBox
BioBam Bioinformatics
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Webinar: Functional annotation and pathway analysis using OmicsBox
967 просмотров · 7 месяцев назад
BioBam Bioinformatics
931 подписчик
967 просмотров · 7 месяцев назад
Learn more about OmicsBox: https://www.biobam.com/omicsbox/
Request a personalized OmicsBox demo here: https://www.biobam.com/book-a-demo/
Practical approaches for generating novel annotations and analyzing pathways of differentially expressed genes.
This webinar demonstrates how to annotate and analyze sequence datasets. The session covers annotation strategies with Blast2GO and presents downstream analysis options, including enrichment analysis, pathway analysis, and popular visualization techniques. A practical OmicsBox demo shows how to use a standard RNA-Seq dataset to transform raw reads into biological insights.
The video features key theoretical concepts alongside a step-by-step practical demonstration using OmicsBox.
Key topics covered in this video:
How to (re)annotate sequence datasets using the Blast2GO methodology.
How to review and combine multiple sources like InterPro and EggNog.
How to perform enrichment analyses, including GSEA and Fisher’s Exact Test.
How to link and visualize expression data to altered pathways from databases like KEGG and Reactome.
Speaker:
Stefan Götz, PhD. (Author of Blast2GO)
CHAPTERS:
00:00 - Welcome and Introduction
01:33 - Webinar Content Overview
03:17 - About BioBam & OmicsBox
19:15 - Transcriptomics & Functional Interpretation Pipeline
20:47 - Case Study 1: Drug Response in a Non-Model Organism
23:13 - Case Study 2: Developmental Transcriptomics
25:19 - Transcriptomics Pipeline Overview
32:49 - Functional Annotation Pipeline
39:52 - Combined Pathway Analysis Theory
43:46 - Demo: Analyzing Dataset 2
44:10 - Demo: Quantification and Differential Expression
48:28 - Demo: Loading Functional Annotation
50:04 - Demo: Functional Enrichment with GSEA
53:06 - Demo: Combined Pathway Analysis
01:01:45 - Demo: Notes on Dataset 1
01:03:43 - Summary & Take-Home Messages
ADDITIONAL RESOURCES:
OmicsBox User Manual & Documentation: https://help.biobam.com/
Read our Blog for more bioinformatics tips: https://www.biobam.com/blog/
Try OmicsBox for free: https://www.biobam.com/trial/
More about Functional Analysis in OmicsBox: https://www.biobam.com/functional-ana...
CONNECT WITH BIOBAM:
• Website: https://www.biobam.com
• LinkedIn: / biobam
• Twitter/X: https://x.com/bio_bam
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#Bioinformatics #FunctionalAnnotation #Blast2GO #OmicsBox #PathwayAnalysis #GSEA #RNASeq #Transcriptomics