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Tissue Culture Series #1: How to Thaw Cells with High Efficiency

MilliporeSigma

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Tissue Culture Series #1: How to Thaw Cells with High Efficiency

27 631 просмотр · 4 года назад
MilliporeSigma
8,53 тыс. подписчиков
27 631 просмотр · 4 года назад
To learn more, visit http://ms.spr.ly/6055w1Wkb Tutorial demonstrating best practices for starting a fresh cell culture from frozen stocks, including cell thawing tips and tricks to maximize survival. • Ice crystals and thaw time • How to avoid osmotic shock • Cryoprotectant removal and media replacement • Thawing stem cells and primary cells • Methods for resuspending cells • Seeding density considerations • Troubleshooting