Filtration method of Sterilization /Depth filters, Membrane filters, Air filters - HEPA & ULPA/Tamil

Filtration is an excellent way to reduce the microbial population in solutions of heat-sensitive material, and sometimes it can be used to sterilize solutions. Filtration process does not destroy but removes the microorganisms. It is used for both the clarification and sterilization of liquids and gases as it is capable of preventing the passage of both viable and non viable particles. Depth filters depend on the density and thickness of the filter to trap particles rather than the pore size. The advantages are- (1) they can retain a large mass of particles before becoming clogged, (2) flow rate of the fluid is high, (3) low cost. Depth filters are commonly used when the fluid to be filtered contains a high load of particles, e.g. industrial applications, such as filtration of food, beverages and chemicals. Membrane filters remove microorganisms by screening them out much as a sieve separates large sand particles from small ones. These filters are used to sterilize pharmaceuticals, ophthalmic solutions, culture media, oils, antibiotics, and other heat-sensitive solutions. There are two important air filters that are used in biological safety cabinets and laminar airflow systems: Laminar flow biological safety cabinets, which employ high-efficiency particulate air (HEPA) filters (a type of depth filter) to remove 99.97% of 0.3 􏳠micrometer in diameter particles. Laminar flow biological safety cabinets or hoods force air through HEPA filters, then project a vertical curtain of sterile air across the cabinet opening. The performance of HEPA filter is monitored by pressure differential and airflow rate measurements. ULPA filters (Ultra-low particulate/penetration air): An ULPA filter can remove from the air at least 99.999% of dust, pollen, mold, bacteria and any airborne particles with a size of 0.12 μm or larger. So, in this video, the filtration method of sterilization process, is clearly explained. To watch the video on dry heat sterilization, click the link below:    • Sterilization by dry heat / Hot air oven /...   To watch the video on moist heat sterilization, click the link below:    • Sterilization by moist heat / Autoclave / ...   To watch the video on radiation method of sterilization, click the link below:    • Physical methods of sterilization / Radiat...