CRISPR Cas9 Screening

Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides an overview of setting up and conducting a large-scale CRISPR screen in the context of Glioblastoma (GBM). Although we focus on GBM here, the concepts are applicable to a wide range of scenarios. Our ongoing CRISPR work is performed as a collaboration between the Sonabend and Ahmed Labs at Northwestern Neurosurgery, supported by NIH/NINDS. For a higher quality version - see https://expl.ai/WBLAHKC Papers and figures referenced: https://www.nature.com/articles/s4146... https://www.cell.com/cell-reports/pdf... https://www.computescotland.com/image... https://www.cell.com/cell/pdf/S0092-8... https://blog.addgene.org/plasmids-101... https://www.thermofisher.com/us/en/ho... https://www.axolbio.com/page/transfec... https://www.mirusbio.com/applications... https://pharmaceuticalintelligence.co...