Inside Bioprocessing: Continuous Perfusion Bioreactors and Cell Culture Design

Perfusion bioreactors enable continuous bioprocessing with higher cell density and productivity. This episode of Inside Bioprocessing explains how continuous perfusion works, how it differs from fed-batch, and what’s required for successful perfusion cell culture. In this episode of Inside Bioprocessing, industry experts discuss continuous perfusion bioprocessing and the growing role of perfusion bioreactors in modern biopharmaceutical manufacturing. Key topics covered in this episode include: 1. What continuous perfusion bioprocessing is and how it works 2. Differences between perfusion, batch, and fed-batch processes 3. Cell retention strategies and perfusion cell culture fundamentals 4. Types of perfusion workflows, including N-1 perfusion and intensified fed-batch 5. Equipment, media, and parameter control required for stable perfusion 6. Challenges in liquid handling, media logistics, and downstream integration 7. The role of automation, data monitoring, and software in long-term perfusion runs The discussion provides a practical view of how perfusion bioreactors are being implemented today and what considerations are critical for success. Explore Thermo Fisher Scientific bioproduction solutions: https://www.thermofisher.com/us/en/ho... 00:09 – Introduction to Continuous Perfusion Bioprocessing 01:30 – What Is a Perfusion Bioreactor? 02:40 – Perfusion vs Batch and Fed-Batch Processes 03:01 – Types of Perfusion Workflows 05:05 – Essential Elements of a Perfusion Process 06:05 – Cell Line Requirements for Perfusion 06:58 – Media Design and Perfusion Rate Optimization 07:58 – Parameter Control and Automation Needs 08:39 – Liquid Handling and Downstream Considerations 12:50 – Common Mistakes When Adopting Perfusion 17:15 – Future Trends in Continuous Perfusion Bioprocessing