CellDiscoverer7 Training, September 2021

Training for Zeiss CD7 Host: Sebastian Barreto Ortiz, Ph.D. 1:56 Start, description of CD7 capabilities 5:06 Description of CD7 Capabilities 6:56 Using the LCD Screen on the CD7 7:40 Live Cell experiments and Using CO2 8:14 Power off procedures 10:41 Power on procedures 13:35 - 15:00 UV sterilization of microscope internals 15:00 Initializing the microscope, Opening Zen 3.4 17:28 Loading samples 20:18 Loading samples: Zen “Sample” Tab 30:07 Zen Software Orientation (Left Side of software) 33:30 Microscope control options (Right Side of software) 35:35 Microscope control: Objective options 45:40 Microscope control: Stage Controls 48:06 Microscope control: Z-axis and focus 49:10 Microscope control: Incubation 51:45 Microscope control: Auto-immersion Controls 56:20 Automatic detection of well-plate format and Stage travel limits 59:37 Setting up workflow and tracks 1:00:20 Workflow: Creating Channels 1:04:45 Workflow: Live/Continuous imaging 1:06:20 Workflow: Focusing and locating sample 1:11:25 Workflow: Setting exposure 1:15:15 Setting up Z-Stacks 1:20:00 Visualizing Z-stack and images (Histogram and visualizing options) 1:22:15 Image Deconvolution 1:24:50 Widefield Oblique imaging 1:30:06 Tile and Position Imaging (imaging multiple wells or locations) 1:41:13 Focus Strategy: Setting up automatic focusing (“Definite Focus” technique) 1:48:24 Focus Strategy: When to use Definite Focus 1:50:00 Focus Strategy: Software Autofocus technique 1:56:24 Setting different focus in various locations 2:00:20 Setting different focus in various locations: Inserting support points for tile regions 2:02:44 Focus strategy: Combine Software Autofocus and Definite Focus 2:04:00 Focus strategy: Use Z-values defined by tiles setup (manual input for each region) 2:10:17 Time Series 2:12:18 Combining Z-Stack, Tiles, and Time Series 2:15:00 Autosave function 2:16:15 Saving experimental workflow 2:19:55 Confocal imaging setup 2:26:35 Airyscan Setup 2:27:00 Airyscan Acquisition 2:28:15 Airyscan Acquisition: Sampling modes (specific to the CellDiscoverer7) 2:31:10 Airyscan Acquisition: Airyscan Detector alignment 2:37:03 Airyscan Z-stack setup 2:41:49 Airyscan Image Processing 2:44:30 Additional Track options (Track switching and detector range) 1:56 Start, description of CD7 capabilities 5:06 Description of CD7 Capabilities 6:56 Using the LCD Screen on the CD7 7:40 Live Cell experiments and Using CO2 8:14 Power off procedures 10:41 Power on procedures 13:35 - 15:00 UV sterilization of microscope internals 15:00 Initializing the microscope, Opening Zen 3.4 17:28 Loading samples 20:18 Loading samples: Zen “Sample” Tab 30:07 Zen Software Orientation (Left Side of software) 33:30 Microscope control options (Right Side of software) 35:35 Microscope control: Objective options 45:40 Microscope control: Stage Controls 48:06 Microscope control: Z-axis and focus 49:10 Microscope control: Incubation 51:45 Microscope control: Auto-immersion Controls 56:20 Automatic detection of well-plate format and Stage travel limits 59:37 Setting up workflow and tracks 1:00:20 Workflow: Creating Channels 1:04:45 Workflow: Live/Continuous imaging 1:06:20 Workflow: Focusing and locating sample 1:11:25 Workflow: Setting exposure 1:15:15 Setting up Z-Stacks 1:20:00 Visualizing Z-stack and images (Histogram and visualizing options) 1:22:15 Image Deconvolution 1:24:50 Widefield Oblique imaging 1:30:06 Tile and Position Imaging (imaging multiple wells or locations) 1:41:13 Focus Strategy: Setting up automatic focusing (“Definite Focus” technique) 1:48:24 Focus Strategy: When to use Definite Focus 1:50:00 Focus Strategy: Software Autofocus technique 1:56:24 Setting different focus in various locations 2:00:20 Setting different focus in various locations: Inserting support points for tile regions 2:02:44 Focus strategy: Combine Software Autofocus and Definite Focus 2:04:00 Focus strategy: Use Z-values defined by tiles setup (manual input for each region) 2:10:17 Time Series 2:12:18 Combining Z-Stack, Tiles, and Time Series 2:15:00 Autosave function 2:16:15 Saving experimental workflow 2:19:55 Confocal imaging setup 2:26:35 Airyscan Setup 2:27:00 Airyscan Acquisition 2:28:15 Airyscan Acquisition: Sampling modes (specific to the CellDiscoverer7) 2:31:10 Airyscan Acquisition: Airyscan Detector alignment 2:37:03 Airyscan Z-stack setup 2:41:49 Airyscan Image Processing 2:44:30 Additional Track options (Track switching and detector range)