(53) Final Paper all Units | Instrumental Methods of Analysis | B.Pharma | Chromatographic Technique

Download the "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Tests and other GPAT Materials - https://play.google.com/store/apps/de... UNIT –I UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, the solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis Fluorimetry Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications UNIT –II IR spectroscopy Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications Flame Photometry-Principle, interferences, instrumentation and applications Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications Nepheloturbidometry- Principle, instrumentation and applications UNIT –III Introduction to chromatography: Adsorption and partition column chromatography-Methodology, advantages, disadvantages and applications. Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications. Paper chromatography-Introduction, methodology, development techniques, advantages, disadvantages and applications Electrophoresis–Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications UNIT –IV Gas chromatography - Introduction, theory, instrumentation, derivatization,temperature programming, advantages, disadvantages and applications High-performance liquid chromatography (HPLC)-Introduction, theory,instrumentation, advantages and applications. UNIT –V Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications. Gel chromatography- Introduction, theory, instrumentation and applications Affinity chromatography- Introduction, theory, instrumentation and applications Get in touch with the solution by just clicking the following links- Facebook Group-   / solutionpharamcy   Mobile App - https://play.google.com/store/apps/de... New Channel (Pharmacy Dictionary)    / @pharmacydictionary   E-Mail for official and other work - solutionpharmacy@gmail.com #solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPAT