Exploring Heterogeneous Cell Populations Using APC and Correlative Imaging - David Colameo

High-throughput automated patch-clamp (HT-APC) is a powerful alternative to manual recordings but is typically restricted to homogeneous cell populations. We established a workflow that combines HT-APC with post-fixation and high-content imaging, allowing the cell type of each patched cell to be identified. As a proof of concept, we created mixed populations with defined electrophysiological and fluorescent properties to validate the method. This approach enables cell-type–specific electrophysiology in complex cultures and primary tissues, opening new possibilities for disease modelling and functional analysis of heterogeneous systems. Visit us at www.nanion.de to learn more about the SyncroPatch 384!