Advances in high-throughput protein workflows and automation challenges | Part 1 | Dr. Edward Kraft

Episode 8 | Dr. Edward Kraft, Leash Bio (Part 1) In this episode, Dr. Andrew Lee and Dr. Edward Kraft (Leash Bio) chat about the intricacies of high throughput protein expression, purification, and characterization. They explore the role of automation in simplifying research processes, discuss various protein expression systems, and consider the merits of different solubility tags and purification techniques. Dr. Kraft shares his career journey, contributions to the field, and thoughts on the biotech industry’s evolving landscape. “Imagine More, Create Solutions” is the podcast for all things biotechnology and life sciences. Hosted by IMCS co-founder and Chief Scientific Officer, Dr. Andrew Lee, the podcast features discussions with leading scientists and experts from various fields including clinical and forensic toxicology, biotech, pharma, academia, and beyond. Whether you’re a scientist, laboratory professional, graduate student, professor, or simply someone who enjoys exploring science in-depth, this podcast is for you. Read more on our website: https://imcstips.com/ Request a free sample of IMCStips here: https://imcstips.com/imcstips/ Show Notes 00:00 Show Intro and Agenda 00:22 Meet Andrew and Edward 02:15 From Farm to Biotech 03:37 High Throughput Book Chapter 05:58 PepTalk and Conference Circuit 09:54 Moving Leash Bio to Salt Lake City 13:34 Avocados and Butter Debate 14:27 Why Protein Expression Is Hard 18:37 Choosing Expression Systems 19:54 BacMam Explained 21:34 Why Skip E coli Sometimes 23:31 Scaling From Small to Large 25:12 Solubility Tag Reality 27:09 Construct Design Tools 29:50 Cell Free Expression Limits 31:44 Modern Purification Tags 34:09 Multipurpose Tagging Ideas 36:53 Automation and Analytics Gaps 40:38 Depth Filtration Upstream 43:37 On Bead Assays Workflow 45:16 Stability Screening Readouts 48:07 Semi-Automated Reality Check 51:24 Closing and Next Steps