Antibody Affinity Purification: Differences Between Proteins A, G, and L

In this video, we’re diving into affinity purification for antibodies—a powerful and widely used technique in protein biochemistry. We’ll break down how affinity chromatography works, review antibody structure (including heavy and light chains), and compare three essential affinity proteins: Protein A, Protein G, and Protein L. #biochemistry #proteinpurification #labtechniques 🔬 Find helpful resources from GoldBio.com below! GoldBio Handbooks - https://goldbio.com/handbooks What is Affinity Purification for Antibodies? - https://www.goldbio.com/blogs/articles/all-about-affinity-purification-of-antibodies Relative Affinity of Immobilized Protein A and Protein G for Various Species and Subclasses of Polyclonal and Monoclonal IgGs - https://www.goldbio.com/blogs/documentation/affinity-comparison-of-various-igg-antibodies Protein Expression & Purification - https://www.goldbio.com/collections/protein-expression-purification Related Products Protein A Magnetic Agarose Beads - https://goldbio.com/products/protein-a-magnetic-agarose-beads Protein G Magnetic Agarose Beads - https://goldbio.com/products/protein-g-magnetic-agarose-beads Protein L Magnetic Agarose Beads - https://goldbio.com/products/protein-l-magnetic-agarose-beads Chapters Intro 0:00 Affinity purification overview 0:35 What is the structure of an antibody 1:32 What are the 5 classes of antibodies 1:59 Difference between Protein A, Protein G and Protein L 2:36 How to chose the right affinity protein? 3:38 For free handbooks visit goldbio.com 4:39 Like the shirt? Enter to win one of your own! - https://goldbio.com/pages/win-a-goldbio-t-shirt _________________________________________________________________________________________ Presented by Karen Martin, Edited by Karen Pierce Gold Biotechnology, Inc. - https://www.goldbio.com At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all. Come say hi on other forms of social media! Linkedin: https://www.linkedin.com/company/gold-biotechnology-inc- Facebook: https://www.facebook.com/GoldBiotechnology Twitter: https://twitter.com/goldbio Instagram: https://www.instagram.com/goldbiostl