Serum Protein Electrophoresis (SPEP) | Patterns, M-Spike & Interpretation Made Easy

A practical guide to Serum Protein Electrophoresis (SPEP) — from the basic principle of protein separation to systematic interpretation of abnormal electrophoretic patterns. The video reviews the major SPEP fractions including albumin, alpha-1, alpha-2, beta and gamma regions, and explains how their patterns change in different clinical conditions. Learn how to recognize monoclonal versus polyclonal gammopathy, an M-spike, acute-phase patterns, beta-gamma bridging, and other important abnormalities. A narrow homogeneous spike suggests a monoclonal protein, whereas broad-based gamma elevation is more typical of a polyclonal response. The video also explains when SPEP alone may not be sufficient and when additional investigations such as serum immunofixation, urine electrophoresis/immunofixation and related monoclonal-protein studies should be considered. Essential for hematology residents, pathology trainees, laboratory professionals and anyone learning the work-up of monoclonal gammopathies and plasma-cell disorders. HemeWise by Dr Sahrish #SPEP #SerumProteinElectrophoresis #MultipleMyeloma #MonoclonalGammopathy #Hematology #HemeWise