Agarose Gel Electrophoresis
Addgene
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Agarose Gel Electrophoresis
337 710 просмотров · 8 лет назад
Addgene
28,4 тыс. подписчиков
337 710 просмотров · 8 лет назад
Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and purification. In this video, we’ll show you how to prepare an agarose gel using powdered agarose and buffer. Then we’ll show you how to load your samples into the gel and perform electrophoresis to successfully separate your DNA bands. We’ll also give you some tips and tricks to get great looking gels every time!
For the full written Agarose Gel Electrophoresis protocol, visit:
https://www.addgene.org/protocols/gel...
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0:00 Intro
0:49 What Percent Agarose to Use?
1:28 Preparing the Gel
3:04 Pouring the Gel
3:34 Loading the Samples and Running an Agarose Gel