How to culture Methanotrophs

Culturing of the model methanotroph Methylmicrobium alcaliphilum 20Z. Visit our lab website for more information on C1 Biocatalysis http://sci.sdsu.edu/kalyuzhlab/ P3% Media: KNO3 1 g/L MgSO4 x 7H2O 0.2 g/L CaCl2 x 2H2O 0.02 g/L NaCl 30 g/L Trace solution (1000x) 1 mL/L Water (distilled deionized water) QS 1L Agar (solid) 15 g/L Phosphate Solution (pH 6.8) KH2PO4 5.44 g/L Na2HPO4 5.68 g/L Carbonate Solution (pH 8.8-9.0) NaHCO3 75.6 g/L Na2CO3 10.5 g/L P media, Trace mineral solution and Phosphate solution should be sterilized by autoclaving @ 121℃ for 30 minutes. Carbonate solution should be sterilized by 0.2µm filtration.