Advanced Unmixing in SpectroFlo (Cytek Aurora)

How to decide between cells controls and beads controls. Video Contents: 0:00 Intro 1:18 Unmix with all beads 2:09 Setting gates on beads in the unmixing wizard 5:56 Setting up worksheets to check unmixing 12:38 Determine which beads controls caused unmixing errors 23:39 Unmixing a second time with a combination of beads and cells 25:37 Setting gates on cells in the unmixing wizard 31:04 Assessing unmixing quality after using beads and cells 31:38 Troubleshooting unmixing errors 35:02 Final checks if there are no unmixing errors 36:00 Applying compensation to make final minor adjustments 39:33 Outro