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Build an HPLC method from Scratch. - wavelength, Solvent and PPM

Chromistrylabs

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Build an HPLC method from Scratch. - wavelength, Solvent and PPM

104 просмотра · 3 недели назад
Chromistrylabs
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104 просмотра · 3 недели назад
Link:    • Method Development- A waste of time?   Welcome to ChromistryLabs! In this episode of our Analytical Method Development series, we dive deep into conversion - How to development hplc method without any literature reference. How to select good detection wavelength. How we can filter unwanted interference. Also we will look into ppm calculation explanation. ​Whether you are working with HPLC, UPLC, GC mastering these fundamental steps is essential for compliance and robust laboratory operations. ​⏱️ Video chapters 0:00 Introduction 1:30 Method reference unavailable 2:00 Importance of wavelength Selection 2:36 Solubility and solvent selection 4:18 Concentration and ppm calculation 12:38 wavelength scanning 13:33 wavelength Selection 15:24 Filter for Interference 18:40 Conclusion ​🔗 Resources & Connect: • Subscribe to ChromistryLabs for more pharmaceutical technical training! • For freelance consulting, technical coaching, or business inquiries, reach out via: https://www.linkedin.com/in/rajesh-mi... ​Drawing on nearly two decades of hands-on experience in analytical development, this channel is dedicated to elevating your laboratory skills and solving complex analytical challenges. ​#AnalyticalDevelopment #ChromistryLabs #HPLC #UPLC #GasChromatography #PharmaTraining #MethodDevelopment #ICH Guidelines #Pharmacopoeia @Chromistrylabs #analytical #pharmaceuticals #pharmacy #laboratory #testing #hplc #chromatography #pharmaguide#peptides