Protein Quantification (OD280)

( http://www.abnova.com ) - Steps of quantifying protein using UV absorbance (280nm) are demonstrated. 280nm absorbance depends on the Tyr and Trp (amino acids with aromatic rings) content. A very rough protein concentration can be obtained by making the assumption that the protein sample has an extinction coefficient of 1 (1 OD = 1 mg/ml protein). More videos at Abnova http://www.abnova.com