DDD group -CNS regionalization and its modeling with in vitro stem cell systems

Developmental Diversity in a Dish: With an introduction from Dr. Ron McKay, we had a group exploration of transcriptomic datasets in CNS regionalization and its modeling in stem cell systems looking at data from previous speakers. We have had 5 talks in the DDD series (https://www.carlocolantuoni.org/seminar) focused on in vitro modeling of CNS regionalization using morphogen exposures in cerebral organoid systems (see listing below). Along with early in vivo brain regionalization data, we are assembling these in vitro data in NeMO Analytics (https://nemoanalytics.org/landing/neo...) as a platform to explore and interact. You can examine the expression of individual genes in these datasets HERE: https://nemoanalytics.org/expression..... In addition to the datasets themselves, we have uploaded gene signatures relevant to regionalization, including morphogen modules from Soraya Scuderi's work (see below) and gene expression signatures of individual patterning centers from Nicola Micali's work in the developing macaque telencephalon (in Science: https://www.science.org/doi/10.1126/s..., the expression of which you can examine in the collection of datasets HERE: https://nemoanalytics.org/projection..... Dr. Hsiu-Chuan Lin (from Dr. Barbara Treutlein's group) "Human neuron subtype programming via single-cell transcriptome-coupled patterning screens" published in Science 10 July 2025: https://www.science.org/doi/10.1126/s... Dr. Akanksha Jain (from Dr. Barbara Treutlein's group): "Morphodynamics of human early brain organoid development" published in Nature 18 June 2025: https://www.nature.com/articles/s4158... Dr. Soraya Scuderi (from Dr. Flora Vaccarino's group) "Specification of human brain regions with orthogonal gradients of WNT and SHH in organoids reveals patterning variations across cell lines" published in Cell Stem Cell 5 June 2025: https://www.cell.com/cell-stem-cell/a... Dr. Neal Amin (from Dr. Sergiu Pasca's group) "Generating human neural diversity with a multiplexed morphogen screen in organoids" published in Cell Stem Cell December 2025: https://www.cell.com/cell-stem-cell/a... Dr. Fátima Sanchis-Calleja (from Dr. Kara McKinley's lab & Dr. Barbara Treutlein's group): "Decoding morphogen patterning of human neural organoids with a multiplexed single-cell transcriptomic screen" on biorxiv: https://www.biorxiv.org/content/10.11...