Methods in Biology : Top 20 Most Repeated PYQs | SET Life Science (MH SET, KSET, MP SET) | IFAS
GATE & SET IFAS: Biotechnology, Life Science & EY
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Methods in Biology : Top 20 Most Repeated PYQs | SET Life Science (MH SET, KSET, MP SET) | IFAS
2 799 просмотров · 8 дней назад
GATE & SET IFAS: Biotechnology, Life Science & EY
44 тыс. подписчиков
2 799 просмотров · 8 дней назад
Methods in Biology in Techniques explained with clear concepts & exam-focused approach. Learn electrophoresis, SDS-PAGE, chromatography, microscopy, PCR, blotting, flow cytometry, FRAP, mass spectrometry & PYQ solving for CSIR NET, GATE & SET.
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Topics Covered in This Lecture :
Electrophoresis, SDS-PAGE, size exclusion chromatography, microscopy, centrifugation, PCR, Northern blotting, flow cytometry, affinity chromatography, ion exchange, FRET, Western blotting, FRAP, apoptosis detection, Sanger sequencing, mass spectrometry
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Summary :
In this session, IFAS Expert Faculty focuses on Methods in Biology through PYQ and MCQ solving. Key concepts include electrophoresis, SDS-PAGE, size exclusion chromatography, differential centrifugation, Beer-Lambert law, Northern blotting, flow cytometry, PCR, SDS-PAGE migration, affinity chromatography, ion exchange chromatography, rate zonal vs isopycnic centrifugation, FRET, Western blotting, FRAP, apoptosis detection, Sanger sequencing, and mass spectrometry. Exam-focused tricks and elimination methods are demonstrated.
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Timestamps :
[0:00] – Introduction
[0:47] – PYQ 1: Electrophoresis (Maharashtra SET 2019)
[1:37] – PYQ 2: SDS-PAGE (Maharashtra SET 2020)
[2:32] – MCQ 1: Size Exclusion Chromatography – Elution Order
[3:15] – MCQ 2: SDS-PAGE Statement
[3:55] – MCQ 3: Microscopy for 3D Surface Morphology (SEM)
[4:23] – MCQ 4: Differential Centrifugation – First Sediment (Nucleus)
[4:53] – MCQ 5: Beer-Lambert Law
[5:20] – MCQ 6: Northern Blotting for mRNA Expression
[5:55] – MCQ 7: Flow Cytometry – Forward vs Side Scatter
[6:35] – MCQ 8: PCR Essential Component
[7:44] – MCQ 9: SDS-PAGE Two Proteins (25 kDa & 75 kDa) Migration
[9:00] – MCQ 10: Affinity Chromatography – Elution Method
[10:02] – MCQ 11: Ion Exchange Chromatography (pI 5 at pH 8)
[10:32] – MCQ 12: Rate Zonal vs Isopycnic Centrifugation
[11:41] – MCQ 13: FRET – Correct Statement
[12:37] – MCQ 14: Western Blotting – Blocking Step Purpose
[13:37] – MCQ 15: FRAP Technique – Conclusion
[16:16] – MCQ 16: Early Apoptotic Cell Staining Pattern
[17:32] – MCQ 17: Sanger Sequencing – Correct Statement
[18:47] – MCQ 18: Mass Spectrometry – Working Principle
[19:50] – End Of Lecture
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