5 Best TLC Staining Agents That Will Help You In Chemistry Lab Every Time! | Dr. Aman
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5 Best TLC Staining Agents That Will Help You In Chemistry Lab Every Time! | Dr. Aman
3 723 просмотра · 3 года назад
Beacon Bin Science
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3 723 просмотра · 3 года назад
If your SM or your product is not UV active then you can’t see them under UV chamber and hence you can’t use this technique. Now to deal with this situation the staining agents comes into picture but before learing about staining agents. To be UV-active, compounds must possess a certain degree of conjugation, which occurs most commonly in aromatic compounds if your compound doesn’t have certain level of double bond conjugation then they are UV inactive.
Fortunately, there are a number of permanent or semi-permanent methods for visualization which will not only allow one to see these compounds but also provide a method for determining what functional groups are contained within the molecule. This method is referred to as staining the TLC plate, and experience will allow you to determine what functional groups will appear as what color upon visualization.
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