How to use Invitrogen LIVE/DEAD Cell Imaging Kit with P3D Scaffolds

NOTE: Error in captions at 01:22. You must add 1mL green staining solution to the 1uL red staining solution. Video protocol for how to perform Cell viability using Invitrogen LIVE/DEAD Cell Imaging Kit with P3D Scaffolds from @Ossiform Research Line. Note: This video is made with customized P3D Scaffolds. Content: 00:13 - Thaw the cell kit at room temperature 00:43 - Aspirate the media from the scaffold 01:09 - Add 200uL media to each scaffold 01:22 - Mix the live/dead cell kit solution by adding 1mL green staining solution to the 1uL red staining solution 02:06 - Add 200uL from the live/dead cell kit solution on the top of each scaffold 02:45 - Cover with tinfoil to prevent it from the light damage and incubate for 15 min. at room temperature 02:59 - Flip each scaffold if the microscope's objective is located at the bottom site of the specimen 03:17 - Evaluate cell viability using flourescent microscope Make sure to check our latest updated protocols on our website: https://ossiform.com/ossiform-researc...