Using ImageJ to measure polyacrylamide gel images

Download ImageJ from the NIH here: https://imagej.nih.gov/ij/download.html 3:30 - How to download ImageJ 5:10 - Installation Windows users 8:30 - Installation for Mac users 11:48 - How to open your TIFF file on ImageJ and set rotation & dimensions 19:10 - Recognize your ladder 28:15 - Using the ROI manager and begin drawing and adding lines 40:01 - Saving and accessing your work 43:13 - Extract measurements and save as a .csv file 48:39 - Open file in Excel and generate your standard curve