How to Leverage Illumina Sequencing in Genome-Wide CRISPR Screens

Learn about how Clustered Regularly Interspaced Short Palindromic repeats (CRISPR) screens can be an important tool in your research, as well as how to perform a successful experiment using NGS as a downstream readout. Presenter: Lauren Fait– Sr. Sequencing Specialist, Illumina 00:00 Introduction 00:32 CRISPR Screening Overview 2:28 Natural CRISPR Systems Have 2 Major Components 5:11 CRISPR Screen Overall Workflow 6:49 sgRNA Library Assembly and Packaging 9:04 NGS Quality Control for Pooled Libraries 10:05 Pooled CRISPR Screen 15:12 Genome-Scale Knockout Libraries 18:00 Hit Identification using Illumina Sequencing 20:58 Single-Cell Sequencing for CRISPR Screens Subscribe to the Illumina video channel http://www.youtube.com/subscription_c... A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos    • Illumina Customer Testimonials   View Illumina webinars    • Webinars   View Illumina product videos    • Плейлист   View Illumina support videos    • Illumina Support