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Gel Electrophoresis Technique | Biotechnology | Dr. Sachin Kapur | NEET | AIIMS.

Dr Sachin Kapur

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Gel Electrophoresis Technique | Biotechnology | Dr. Sachin Kapur | NEET | AIIMS.

515 просмотров · 4 нед. назад
Dr Sachin Kapur
179 тыс. подписчиков
515 просмотров · 4 нед. назад
🎓 GEL ELECTROPHORESIS | Complete Concept Explained | Dr. Sachin Kapur How can we separate DNA fragments of different sizes and actually see them in the laboratory? 🧬🔬 This detailed video, explains the Gel Electrophoresis Technique from the basics to the complete working principle, making this important Biotechnology concept easy to understand for NEET aspirants and Biology students. 🔬 In this video, we will understand: ✅ What is Gel Electrophoresis? ✅ Why is electrophoresis required for DNA separation? ✅ Why does DNA move towards the anode (+)? ✅ Role of the negative charge of DNA ✅ Why is agarose gel commonly used for DNA separation? ✅ How is the agarose gel prepared? ✅ What are wells and how is DNA loaded into them? ✅ Role of the electrophoresis buffer ✅ How an electric field separates DNA fragments ✅ Why do smaller DNA fragments move faster and farther than larger fragments? ✅ What is meant by DNA ladder / molecular marker? ✅ How are separated DNA fragments visualised? ✅ Role of staining dyes in DNA visualisation ✅ How can we determine the approximate size of an unknown DNA fragment? ✅ How gel electrophoresis is used in Recombinant DNA Technology ✅ Applications of gel electrophoresis in biotechnology, molecular biology and forensic science The lecture also connects the technique with the NCERT Biotechnology: Principles and Processes chapter so that students can understand not just what happens, but why it happens. 🧬 Why is Gel Electrophoresis important? Gel electrophoresis is one of the fundamental techniques of molecular biology. It allows scientists to separate DNA fragments according to their size, analyse DNA samples and identify fragments of interest. Whether you are studying Recombinant DNA Technology, PCR, DNA fingerprinting, cloning or molecular diagnostics, understanding gel electrophoresis is essential. 👨‍🏫 Lecture by: Dr. Sachin Kapur Biology Educator | NEET Biology | Biotechnology & Molecular Biology 📌 For NEET aspirants, Biology teachers and students who want to understand Biotechnology conceptually rather than simply memorising it. If you find this lecture useful, LIKE 👍 | SHARE 📲 | SUBSCRIBE 🔔 to the channel for more detailed Biology and Biotechnology lectures. #GelElectrophoresis #DrSachinKapur #Biotechnology #MolecularBiology #DNA #RecombinantDNATechnology #NEET2027 #NEETBiology #Biology #AgaroseGel #DNASeparation #GeneticEngineering #PCR #DNAFingerprinting #BiologyLecture