Northern Blot RNA Quantification Step by Step | ImageJ Alternative

Northern blot remains the gold standard for RNA size and splice variants. Phoretix 1D quantifies bands and sizes RNA without manual ImageJ densitometry. Try it on your own images in your own lab for free now: https://totallab.com/software/free-we... This tutorial covers band detection on autorad, phosphor or chemiluminescent Northerns, RNA size calibration from an RNA ladder, transcript abundance quantification, and reporting. What you'll learn: 00:14 – Introduction 01:38 – Identifying saturated areas in an image 00:52 – Results of automatic analysis 03:20 – Drawing lanes 04:11 – Editing lanes to match band 05:00 – Background subtraction 06:10 – Assigning a molecular weight ladder 06:24 – Designing a new molecular weight ladder template 07:10 – Assigning molecular weight curve fitting algorithm 08:00 – Detecting RNA bands 08:09 – Manually editing bands 12:06 – Outro and free trial link IDEAL FOR Splice variant confirmation studies mRNA size verification post-cloning miRNA / small RNA Northern workflows Plant molecular biology (where Northern remains widely used) Teaching labs covering classical molecular biology techniques KEYWORDS Northern blot analysis, Northern blot quantification, RNA gel analysis, mRNA quantification, RNA size determination, splice variant analysis, miRNA Northern, RNA ladder calibration, RNA densitometry, qPCR alternative confirmation, Northern blot software #NorthernBlot #RNA #GelAnalysis