Biochemistry techniques - overview of some biochem, molecular biology, & structural biology methods

Here are some of the many many various techniques used in biochemistry, molecular, and structural biology. At this link: http://bit.ly/labtechniques you can find more details about them all. Here I just want to let you know they exist and give you an idea of the types of experiments you can use to ask molecules various questions.  P.S. This video got pretty long - and I still didn’t get into the protein purification stuff! So I added timestamps to help you skip to what you want and also am planning to do another video overviewing protein expression & purification techniques (hopefully that will be of help to some - and hopefully this one will be of help to some!). And sorry the graphics are small on the screen but if you go to the posts you can see lots of full-size ones   I introduce the fundamentals like gels (polyacrylamide & agarose), PCR, & molecular cloning, as well as various more obscure techniques like kinase assays and slot blot binding assays I just happen to have experience with.    Key takeaway - there are lots of experimental techniques and you probably won’t be doing them all, but it’s good to know how they work so you can turn to them if you need them, and so you can interpret results from them in papers. There are also usually multiple ways to get answers to different questions, although you might learn slightly different things depending on which approach you choose. But you need to know what approaches you can choose - hence this post.   Also note that there are a lot of other techniques I didn’t even get into but hope this can serve as a launch point for you to explore and discover more.   0:00 overview 6:44 gel electrophoresis 15:07 blots 19:32 working with nucleic acids 27:18 measuring “expression” 35:00 manipulating expression, gene/mRNA/protein levels, etc. 44:48 detecting and measuring interactions 52:47 molecular cloning 1:02:44 measuring concentrations 1:03:37 experimental design & considerations 1:08:47 structural biology 1:15:38 lab fundamentals 1:17:08 other random things 1:17:46 protein expression and purification   lab techniques: blog page: https://thebumblingbiochemist.com/let... YouTube playlist:    • lab techniques and equipment   practical lab tips & tricks: https://bit.ly/lab_tricks_page &    • Practical lab tips & tricks   protein expression & purification: blog page: https://thebumblingbiochemist.com/let... YouTube playlists recombinant protein expression:    • Recombinant protein expression   protein purification:    • Protein chromatography   protein chromatography:    • Protein chromatography   molecular cloning:    • molecular cloning   structural biology: blog page: https://bit.ly/structural_biology YouTube:    • Structural biology   more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com