Chiral, Ion-Pair & IonChromatography

In Episode 27 of "Chromatography Mastery," we step away from standard C18 columns to explore three specialist techniques designed to conquer the most stubborn separations: Chiral, Ion-Pair, and Ion Chromatography. ⚡ What You'll Learn in This Video: • How chiral stationary phases work and why separating enantiomers is critical for drug safety. • Calculating Enantiomeric Impurity and Enantiomeric Excess (ee). • Using Ion-Pair Chromatography to retain highly polar bases that won't stick to C18. • The four golden rules of Ion-Pairing: equilibration, temperature, dedicated columns, and LC-MS limits. • How Ion Chromatography (IC) and suppressed conductivity detect invisible inorganic counter-ions. • A real lab calculation: determining chloride content in a hydrochloride salt. ⏱️ Timestamps: 0:00 - The Three Separations a C18 Column Can't Handle 0:42 - Enantiomers, Thalidomide, and ICH Q6A 1:32 - How Chiral Stationary Phases & Selectors Work 2:20 - The Three Modes of Chiral Chromatography & Bench Rules 3:15 - Lab Walkthrough: Calculating Enantiomeric Impurity & ee 4:15 - Ion-Pair Chromatography: Retaining Small, Polar Bases 4:55 - The Four Golden Rules of Ion-Pairing 5:40 - Ion Chromatography (IC) for Counter-Ions (Chloride, Sodium) 6:10 - How Suppressed Conductivity Detection Works 6:50 - Lab Walkthrough: Calculating Chloride Content in a Salt 7:45 - How to Choose the Right Specialist Technique ⏭️ Next Video (Ep 28): Size Exclusion & Biopharma Chromatography — Subscribe to PharmaCores and follow the "Chromatography Mastery" playlist to learn how we separate massive biomolecules based purely on their size in solution. #ChiralChromatography #IonChromatography #HPLC #AnalyticalChemistry #PharmaCores