HPLC Detectors UV PDA FLD RID

In Episode 19 of "Chromatography Mastery," we explore the different types of HPLC detectors and how to choose the right one when a standard UV detector just won't work. ⚡ What You'll Learn in This Video: • How UV and PDA detectors use the Beer-Lambert law for reliable quantitation. • Using PDA spectral data to check for peak purity and confirm identity. • Why Fluorescence (FLD) offers unmatched sensitivity for specific compounds. • The strengths and strict limitations of the Refractive Index Detector (RID). • How aerosol detectors (ELSD and CAD) provide universal detection for gradient methods. • A practical walkthrough on selecting the right detector for a non-chromophoric excipient. ⏱️ Timestamps: 0:00 - Introduction: When UV Fails (The Sorbitol Problem) 0:45 - The Four Questions to Ask When Choosing a Detector 1:10 - UV Detectors & The Beer-Lambert Law 1:55 - PDA Detectors: Spectra, Peak Purity & Hidden Settings 3:15 - Fluorescence Detectors (FLD) & Sensitivity 3:55 - Refractive Index Detectors (RID): Pros & Limits 4:45 - Evaporative Light Scattering Detectors (ELSD) 5:30 - Charged Aerosol Detectors (CAD) 6:15 - Lab Walkthrough: Choosing a Detector for Sorbitol ⏭️ Next Video (Ep 20): System Suitability Testing — Subscribe to PharmaCores and follow the "Chromatography Mastery" playlist to learn how to prove your entire system is fit for use before you inject your first sample. #HPLCDetectors #AnalyticalChemistry #Chromatography #QualityControl #PharmaCores