Apoptosis Techniques | Developmental Biology | CSIR DBT JRF 2026 | Lec-34 | IFAS

Apoptosis Techniques | Developmental Biology | CSIR DBT JRF 2026 | Lec-34 | IFAS explained with clear concepts and exam-focused approach. Master apoptosis techniques, necrosis vs apoptosis, annexin V, TUNEL, FACS analysis tricks for CSIR DBT JRF 2026. Covers PYQ concepts, key markers, and detection methods to boost problem-solving. 🔥 Join India's Best Life Science Coaching - IFAS Online! 🔥 Exclusive Crash Live Course Batch: Starts on 01 June 2026 🔥 Live Online Batch: Super Emotion Batch 7.6 - Starts on 18 May 2026 🔥 Recorded Online Batch: Revolution Batch 7.6 from Live Batch Completed on 25 April 2026 📞 Need Guidance? Call/WhatsApp: 9172266888 ━━━━━━━━━━━━━━━━━━━━━━━━━ ⏳ Lecture Timeline: [00:00:00] – Introduction to Apoptosis Techniques [00:06:30] – Types: Necrosis vs Apoptosis [00:08:02] – Necrosis: Accidental Cell Death [00:08:34] – Apoptosis: Programmed Cell Death [00:13:06] – Autophagy: Self‑eating (Brief) [00:14:06] – Morphological Changes in Apoptosis [00:19:51] – Membrane Blebbing & Apoptotic Bodies [00:24:19] – Diagram: Necrosis vs Apoptosis [00:34:24] – Detection Methods Overview [00:34:38] – Detection: Phosphatidylserine (PS) Flipping [00:36:55] – Trick: PS as "Eat Me" Signal [00:38:30] – Annexin V‑FITC Assay [00:43:46] – Detection: DNA Ladder (Fragmentation) [00:44:40] – ICAD/CAD & Caspase Activation [00:50:15] – Detection: TUNEL Assay (TdT) [00:58:37] – Detection: Mitochondrial ΔΨm Loss [01:08:15] – ΔΨm Detection by Cationic Dyes [01:12:46] – Detection: FACS (Flow Cytometry) [01:14:37] – FACS Staining: Annexin V & PI [01:23:01] – Quadrant Interpretation (Healthy/Necrotic/Early/Late) [01:29:47] – Question: FACS Axis Swap [01:33:03] – Answer: Quadrants after Swap [01:36:11] – DPP Assignments [01:44:04] – Complete Session (Apoptosis & Detection Methods) ━━━━━━━━━━━━━━━━━━━━━━━━━ 📖 Topics Covered in This Lecture: • Necrosis vs apoptosis (programmed cell death) concepts • Phosphatidylserine flipping & annexin V staining tricks • DNA fragmentation: agarose gel ladder & TUNEL assay • Mitochondrial membrane potential detection PYQ problems • FACS/FACS analysis for apoptotic cell sorting ━━━━━━━━━━━━━━━━━━━━━━━━━ 📚 Lecture Summary: In this session, IFAS Expert Faculty focuses on apoptosis techniques, starting with necrosis vs apoptosis, morphological changes, phosphatidylserine externalization and annexin V detection. DNA fragmentation analysis via agarose gel laddering and TUNEL assay, mitochondrial membrane potential changes, and FACS-based quantification are explained with crisp tricks. The apoptosis techniques lecture links every method to CSIR DBT JRF exam patterns and direct PYQ-style problem-solving approaches for quick revision. ━━━━━━━━━━━━━━━━━━━━━━━━━ 🎓 This lecture is highly beneficial for students preparing for: ✅ CSIR NET Life Science ✅ GATE Life Science ✅ GATE Biotechnology ✅ GATE Ecology & Evolutuon ✅ BET (DBT) ✅DHR ICMR (BRET) ✅ TIFR Biology  ✅ BARC Bioscience  ✅ SET / PhD Entrance Exams ━━━━━━━━━━━━━━━━━━━━━━━━━ 🏆 Why IFAS is India’s No. 1 Choice for CSIR NET & GATE Life Science? (Our Results Speak): ✅ CSIR NET Dec 2025: 4000+ Selections (AIR 3, 8, 9, 9...) ✅ CSIR NET June 2025: 4200+ Selections (AIR 1, 2, 6, 10...) ✅ CSIR NET Dec 2024: 4000+ Selections (AIR 5, 6, 7, 8, 9...) ✅ CSIR NET June 2024: 2000+ Selections (AIR 3, 10, 13...) ✅ CSIR NET Dec 2023: 1200+ Selections (AIR 2, 4, 5...) 📄 View IFAS Result Sheet: 👉 https://drive.google.com/file/d/1THh6... ━━━━━━━━━━━━━━━━━━━━━━━━━ 🔗 Important Links: 🌍 Visit our Website: https://ifasonline.com 📞 Contact for Queries: 9172266888 📘 Free Counselling: https://ifasonline.com/csir-net/life-... ━━━━━━━━━━━━━━━━━━━━━━━━━ 🔗 India's Best Online Classes and Books: 🔥CSIR NET Life Science Live & Recorded Course: https://ifasonline.com/csir-net/life-... 🎯 GATE Life Science Recorded Course: https://ifasonline.com/gate/life-scie... 🎯 GATE Biotechnology Recorded Course: https://ifasonline.com/gate/biotechno... 📘CSIR NET Life Science Books: https://ifasonline.com/csir-net/life-... ━━━━━━━━━━━━━━━━━━━━━━━━━ 📲 Download IFAS App: Android: https://play.google.com/store/apps/de... IOS: https://apps.apple.com/us/app/ifas/id... ━━━━━━━━━━━━━━━━━━━━━━━━━ Stay Updated Via Other Social Media: ✔ Telegram: https://t.me/IfasLifeScience ✔ Instagram:   / csir_net_life_science_ifas   #apoptosistechniques #developmentalbiology #csirnetlifescience #gatelifescience #gatebt #setlifescience