Understanding Distance Transformations and using them in CellProfiler: AskErinDearBeth Episode 41

In this episode, Erin and Beth talk about Distance Transformations and demonstrate how to use them in CellProfiler. Distance Transforms are a helpful tool to use during image analysis and Erin shows off two different potential use-cases using the DistanceTransform module in CellProfiler: measuring distances (and overlap) between objects of one category versus another in order to quantify complex spatial relationships. This is especially used for tissue images measuring the distance of an object from the physical edge of a well in a multi-well plate so that QC can be performed outside of the pipeline to programmatically filter out cells that are "too close" to the well edge #science #openscience #microscopy #tutorial #opensource #biology #imageanalysis #segmentation #cellprofiler ================================================== On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s about tuning or troubleshooting, segmenting or measuring, Beth and Erin have opinions and by golly are they going to share them! Using mostly CellProfiler, but with expertise in many other image analysis softwares such as ImageJ/FIJI, Cellpose, Piximi, and more, no image analysis question is too hard, so ask away! Submit your own questions at broad.io/AskErinDearBeth Images and pipelines we’ve used are available for you to download and play with at https://ciminilab.github.io/AskErin_D...