Running (and setup) of your first method, SEC
Unicorn Stuff
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Running (and setup) of your first method, SEC
1 011 просмотров · 3 года назад
Unicorn Stuff
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1 011 просмотров · 3 года назад
This video will show you step-by-step how to setup and run your first SEC method in Unicorn on the AKTA Avant. See my other video where I show you how to write the preset method we use here.
Size Exclusion Chromatography (SEC) is a way to separate macro-molecules based on size (mostly). Here we setup a couple runs for purification using a column of S200 resin (or media) from Cytiva (GE Healthcare).
For more reading on creating methods: https://cdn.cytivalifesciences.com/ap...
Getting your system started for a run: https://b3p.it.helsinki.fi/download/A...
See detailed video on how to prime your AKTA's pumps: • Pump Wash and AKTA Avant
Reach me at my Linkedin page / chad-blamey-ph-d-649a336
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