Immune Binding & Kd: Sickle Cell, TLRs, Antibodies, MHC | Stryer Biochemistry Ch 3 Explained, Part 2

Binding and Molecular Recognition: the immune system, antibodies and binding constants. Stryer Biochemistry Chapter 3, Part 2 of 2, explained topic by topic with the book on screen and 3D molecular pictures. The second half of Chapter 3: why low pH and CO2 make hemoglobin release oxygen, how one amino acid change causes sickle-cell anemia, and how Toll-like receptors, antibodies and MHC proteins recognise invaders. Then binding in numbers: Ka and Kd, equation 2, competition between ligands, rate constants and single molecules. Every equation is derived step by step, and the worked example and all self-check questions are solved. Chapters: 0:00 Chemical basis of the Bohr effect 4:04 The effect of carbon dioxide 8:14 Carbon monoxide and neuroglobin 12:16 Sickle-cell anemia 16:33 How hemoglobin S fibers form 20:22 The immune system: self vs non-self 23:35 Toll-like receptors 26:57 TLR3 recognises double-stranded RNA 30:53 Antibodies: hypervariable regions 34:43 The immunoglobulin fold 37:45 IgG is Y-shaped 40:55 Papain cuts IgG: Fab and Fc 43:53 An antibody binds lysozyme 48:17 Binding units and effector units 52:33 Recombination and vaccines 55:58 Class I MHC: cut and display 59:34 Anchor residues 1:02:51 T-cell receptors, self vs non-self 1:07:13 Equilibrium constants: Ka and Kd 1:11:11 Kd equals L1/2, and equation 2 1:15:16 Using equation 2: Figure 3.49 1:18:24 Worked example: fraction bound 1:21:38 Specificity: comparing Kd values 1:26:54 Kinetics: k1, k-1 and Kd 1:31:32 Real rate constants 1:35:14 Tamoxifen and single molecules 1:40:06 Summary and key terms Watch Part 1 first: binding basics, myoglobin, hemoglobin, cooperativity, T and R states and 2,3-BPG. Book: Biochemistry (Berg, Tymoczko, Gatto, Stryer), 10th edition, Chapter 3. If you like this video, please comment and let us know. We will continue with the next chapters. Tell us in the comments which chapter you want next! Like, share and subscribe to support the channel. #Biochemistry #Stryer #Antibodies #Immunology #SickleCell #TollLikeReceptors #MHC #Kd #EnzymeKinetics #MScBiology