How to Calculate Transformation Efficiency

Learn how to calculate genetic transformation efficiency with this whiteboard video. * Note, in this video we reference a "second dilution" the second dilution being referenced isn't a formal dilution. Instead, it is the plating of the cells. For ease, it's been referred to as Dilution #2 or a second dilution* Transformation efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid into a given volume of competent cells. TE = Colonies/μg/Dilution Where: Colonies = the number of colonies counted μg = amount of DNA transformed in μg Dilution = total dilution of the DNA before plating Transformation Efficiency Calculator - https://www.goldbio.com/pages/transfo... Products: GB10B™ Chemically Competent E. coli Cells: https://goldbio.com/products/gb10b-ch... GoldBio's Competent Cells - https://www.goldbio.com/collections/e... Related articles/resources: Introduction to Competent Cells - https://www.goldbio.com/blogs/article... A Quick Overview of Molecular Cloning - https://www.goldbio.com/blogs/article... GB10B™ Chemically Competent E. coli Cells Transformation Protocol - https://www.goldbio.com/blogs/documen... GoldBio's Competent Cell Guide - https://www.goldbio.com/blogs/documen... _________________________________________________________________________________________ Gold Biotechnology, Inc. - https://www.goldbio.com At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all. Come say hi on other forms of social media! Linkedin:   / gold-biotechnology-inc-   Facebook:   / goldbiotechnology   Twitter:   / goldbio   Instagram:   / goldbiostl